PD-L1 antibody (AA 25-241)

Catalog No. ABIN7112475

This anti-PD-L1 antibody is a Mouse Monoclonal antibody detecting PD-L1 in WB, IHC, ELISA and IF. Suitable for Human, Mouse and Pig.

Quick Overview for PD-L1 antibody (AA 25-241) (ABIN7112475)

Target: PD-L1 (CD274 (PD-L1))

Reactivity: Human, Mouse, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This PD-L1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Clone: 6C11

Product Details anti-PD-L1 Antibody

Binding Specificity: AA 25-241

Purpose: CD274 antibody

Purification: Protein A+G purification

Purity: ≥95 % as determined by SDS-PAGE

Immunogen:

Immunogen sequence: 25-241 aa

Immunogen: CD274 molecule

Isotype: IgG1

Application Details

Application Notes: WB: 1:500-1:5000, IHC: 1:100-1:500, IF: 1:50-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for PD-L1

Target: PD-L1 (CD274 (PD-L1))

Alternative Name: CD274

Background:

Synonyms: Programmed cell death 1 ligand 1 (PD-L1, PDCD1 ligand 1, Programmed death ligand 1, hPD-L1)|B7 homolog 1 (B7-H1)|CD274|B7H1|PDCD1L1|PDCD1LG1|PDL1

Background: Programmed cell death ligand 1(CD274, or B7-H1, PD-L1), is the first member of B7 family to be discovered. B7 family molecules are type I transmembrane proteins belonging to the immunoglobulin superfamily. In concert with their CD28 family receptors, the B7s are key regulators of the adaptive immune response. CD274 is suggested a negative regulator of T and B cell, and play important role in mediating tolerance of lymphocytes to self-antigens. It also involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. PD-L1 is a 290 aa transmembrane protein with a calculated molecular weight of 33 kDa, the apparent molecular weight has been reported as 45-70 kDa, suggesting probable glycosylation(PMID: 18760278, 16493058).?

Molecular Weight: 40-50 kDa

Gene ID: 29126

UniProt: Q9NZQ7

Pathways: Cancer Immune Checkpoints